Selective Ultratrace Detection of Al(III) and Ga(III) Complexed with a Calcein Isomer by Capillary Zone Electrophoresis with Laser-induced Fluorescent Detection

1 2 3 4 5 6 7 8 9 10 11 A highly sensitive and selective capillary zone electrophoresis with laser-induced fluorescent detection (CZE-LIF) for Al and Ga was developed using a calcein 4’,5’-isomer as a pre-capillary complexing agent. Direct fluorescent detection without an agent added to the carrier buffer provided the notable detection limits of 3.9 × 10 and 4.6 × 10 mol dm for Al and Ga, respectively. By applying a river water sample successfully to this system, its potential for practical applications was ascertained. A comparison of three aromatic polyaminocarboxylate ligands related to the coordination structure and the distance between the fluorophore and the chelating moiety, revealed some interesting factors regarding their selectivity.